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Product Description
Human Proteinase 3 Antibody (PR3Ab) ELISA Kit | AE62523HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: PR3Ab
Alternative Name: N/A
Application: ELISA
Range: 0.156-10 ng/mL
Sensitivity: 0.061 ng/mL
Intra-Assay: ≤4.8%
Inter-Assay: ≤8.3%
Recovery: 1
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Competitive ELISA
Analysis Method : Qualitative
Test Principale: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PR3Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated PR3Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled PR3Ab and unlabeled PR3Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PR3Ab in the sample. The color development is stopped and the intensity of the color is measured.
Product Overview: PRTN3 is a serine protease enzyme expressed mainly in neutrophil granulocytes. Its exact role in the function of the neutrophil is unknown but in human neutrophils proteinase 3 contributes to the proteolytic generation of antimicrobial peptides. It is also the epitope of anti-neutrophil cytoplasmic antibodies (ANCAs) of the cANCA (cytoplasmic subtype) class, a type of antibody frequently found in the disease Wegener's granulomatosis.Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
