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Product Description
Human Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (PDHA1) ELISA Kit | AE28204HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: PDHA1
Alternative Name: RP11-723P2.1; PDHA; PDHCE1A; PHE1A; PDHE1-A type I|pyruvate dehydrogenase E1 alpha 1|pyruvate dehydrogenase E1 alpha subunit|pyruvate dehydrogenase E1 component subunit alpha; somatic form; mitochon
Application: ELISA
Range: 0.156-10 ng/mL
Sensitivity: 0.063 ng/mL
Intra-Assay: ≤5.1%
Inter-Assay: ≤9.6%
Recovery: 0, 86
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PDHA1 in samples. An antibody specific for PDHA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDHA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDHA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDHA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1) ; dihydrolipoyl transacetylase (DLAT) (E2) ; and dihydrolipoyl dehydrogenase (DLD) (E3) . The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex .Pyruvate dehydrogenase (E1) performs the first two reactions within the pyruvate dehydrogenase complex (PDC) : a decarboxylation of substrate 1 (pyruvate) and a reductive acetylation of substrate 2 (lipoic acid) .
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
