Biovision | Androsterone ELISA Kit | E5112-100
Description: Androsterone, or 3α-hydroxy-5α-androstan-17-one, is an endogenous steroid hormone, neurosteroid, and putative pheromone. An androgen is any natural or synthetic steroid hormone that regulates the development and maintenance of male characteristics in vertebrates by binding to androgen receptors. It is a weak androgen with a potency that is approximately 1/7 that of testosterone. Androsterone has generally been considered to be an inactive metabolite of testosterone, which when conjugated by glucuronidation and sulfation allows testosterone to be removed from the body, but it is a weak neurosteroid that can cross into the brain and could have effects on brain function. Androsterone is also known to be an inhibitory androstane neurosteroid, acting as a positive allosteric modulator of the GABAA receptor and possesses anticonvulsant effects. BioVision’s Androsterone ELISA Kit is used to quantitatively measure Androsterone in Serum, pa and other biological fluids. The kit is based on the Competitive ELISA principle. The micro-ELISA plate provided in this kit has been pre-coated with Androsterone. During the reaction, Androsterone in samples or Standard competes with a fixed amount of Androsterone on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Androsterone. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Androsterone in the samples can be calculated by comparing the OD of the samples to the standard curve.
Alternate Name: 3α-hydroxy-5α-androstan-17-one ELISA Kit, 5α-androstan-3α-ol-17-one ELISA Kit; Androsterone ELISA Kit; Inhibitory astane neurosteroid ELISA Kit
Tag Line: A Competitive ELISA kit for in vitro quantitative determination of Androsterone concentrations in serum, plasma and other biological fluids.
Detection Method: Absorbance is measured at 450 nm
Sample Type: Serum, plasma and other biological fluids
Species Reactivity: All
Features & Benefits: Detection Range: 0.78-50 ng/ml Sensitivity: 0.47 ng/ml Highly sensitive and specific