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Product Description
Cleaved-DFF45 (D224) polyclonal Antibody | BS7010 | Bioworld
Host: Rabbit
Reactivity: Human,Mouse,Rat
Application: WB
Application Range: WB: 1:500~1:1000
Background: The CED/ICE family of cysteine proteases plays a pivotal role in mediating apoptosis through the proteolysis of specific targets. Among the targets are poly (ADphospho-ribose) polymerase (PARP), gelsolin, DFF-45/ICAD and the nuclear lamins. PARP is a nuclear protein that is specifically cleaved by CPP32 and Mch2, but not by ICE, into a signature apoptotic fragment. Gelsolin is cleaved by CPP32 to an active form that severs actin filaments in a Ca2+-independent manner. In addition to binding actin, gelsolin can form complexes with fibronectin, which may be important for localizing gelsolin to inflammatory sites. DFF-45/ICAD, the subunit of DNA fragmentation factor, is cleaved by CPP32 to generate an active factor that induces DNA fragmentation. The nuclear Lamin A is cleaved by Mch2, but not CPP32. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family
Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Cleaved-DFF45 (D224) polyclonal Antibody detects endogenous levels of Cleaved-DFF45 (D224) protein.
Molecular Weight: ~ 35, 45 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Names: Endonuclease 8-like 3; DNA glycosylase FPG2; DNA glycosylase/AP lyase Neil3; Endonuclease VIII-like 3; Nei-like protein 3; NEIL3
Immunogen: Synthetic peptide, corresponding Human NEIL3.
Conjugate: Unconjugated
Modification: Cleaved
Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Pathway:
