Product Description
Human TMEM48 (Transmembrane protein 48) ELISA Kit | ELK8813 | ELK Biotechnology
Alternative Names: hNDC1; NDC1 transmembrane nucleoporin; NET3; Nuclear division cycle 1 homolog; TMEM48; Transmembrane protein 48
Species: Human
Assay Type: Sandwich
Sensitivity: 11.5 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Cytokine;Neuro science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transmembrane protein 48 (TMEM48) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Transmembrane protein 48 (TMEM48) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transmembrane protein 48 (TMEM48), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transmembrane protein 48 (TMEM48) in the samples is then determined by comparing the OD of the samples to the standard curve.