Product Description
ELP3 Antibody (N-term) [APR30732G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H, M
Specificity: This ELP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-44 amino acids from the N-terminal region of human ELP3.
Cellular Localisation: Cytoplasm. Nucleus [Isoform 2]: Cytoplasm. Nucleus
Molecular Weight: 62259
Clone: Polyclonal
Gene Name: ELP3 {ECO:0000303|PubMed:15902492, ECO:0000312|HGNC:HGNC:20696}
Gene ID: 55140
Function: Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation (PubMed:11714725, PubMed:11818576, PubMed:15902492, PubMed:16713582) . The elongator complex is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5- carbamoylmethyl uridine) (PubMed:29415125) . In the elongator complex, acts as a tRNA uridine (34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity) . May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins; such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase (PubMed:29415125) . Involved in neurogenesis: regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation (PubMed:19185337) . Required for acetylation of GJA1 in the developing cerebral cortex (By similarity) .
Summary: Tissue Location: Expressed in the cerebellum and spinal motor neurons.
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: WB
Dilution: WB--1:1000
Synonyms: Elongator complex protein 3, hELP3, ELP3