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Product Description
STK35 Antibody | 46-445 | ProSci
Host: Goat
Reactivity: Human, Mouse, Pig
Homology: Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen: The immunogen for this antibody is: ELETRMDQVTCAA
Research Area: Cancer
Tested Application: E, WB, IHC, IF, Flow
Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 55kDa band observed in Human Breast cancer and in Mouse and Pig Testis lysates, and approx. 58kDa in nuclear lysates of cell line HEK293 (calculated MW of 58.1kDa according to Human NP_543026.2, 59.5kDa according to Mouse NP_899085.3 and 59.2kDa according to XP_005672850.1 Pig XP_005672850.1) . Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. This product has been successfully used in WB on Human: Goyal P et al, PLoS One. 2009 Sep 16;4 (9) :e6981. PMID: 19756140.Immunohistochemistry:In paraffin embedded Human Testis shows granular cytoplasmic staining of Leydig cells. Recommended concentration: 10ug/ml.Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
Specificiy: N/A
Positive Control 1: Cat. No. 1313 - Human Testis Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: STK35, Clik1, bA550O8.2, bA550O8.2 CLIK1, serine/threonine kinase 35, CLP-36 interacting kinase, CLIK1, STK35L1, OTTHUMP00000030006, serine threonine kinase 35 long form
User Note: N/A
BACKGROUND: N/A
