QuantiChrom™ Hemoglobin Assay Kit

Application: For quantitative determination of hemoglobin and evaluation of drug effects on hemoglobin metabolism.

Key Features:

• Sensitive and accurate. Linear detection range 0.9 - 200 mg /dL hemoglobin in 96-well plate assay.
• Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.
• Safety. Reagents are non-toxic.
• Versatility. Assays can be executed in 96-well plate or cuvet.

Method: OD400nm

Samples: Blood, serum, plasma, urine etc

Species: All

Size: 250 tests

Detection Limit: 0.9 mg/dL

Shelf Life: 12 months

More Details:

HEMOGLOBIN (Hb) is made of four globin chains each carrying a heme group. It is carried by red blood cells and transports oxygen from the lungs to the peripheral tissues to maintain the viability of cells. Quantitation of blood hemoglobin has been a key diagnostic parameter for various diseases such as anemia, polycythemia and dehydration.

Simple, direct and automation-ready procedures for measuring hemoglobin concentration are becoming popular in Research and Drug Discovery. BioAssay Systems QuantiChrom™ hemoglobin assay kit is based on an improved Triton/NaOH method, in which the hemoglobin is converted into a uniform colored end product.

The intensity of color, measured at 400 nm, is directly proportional to hemoglobin concentration in the sample. The optimized formulation exhibits high sensitivity and is ideal for measuring hemolysis in low hemoglobin samples (e.g. serum and plasma).