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Product Description
Human IL-9 protein, His Tag | PRP100272 | Abbkine
Product Category: Cytology
Application: Cytokines
Product Type: Cytokines
Sequence: Amino acid sequence derived from human IL-9 (NP_000581.1) (Gln 19-IL-4 144) was expressed and purified, fused with a polyhistidine tag at the C-terminus.
Activity: Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 0.5-3 ng/mL.
protein Lenght: The recombinant human IL-9 consists of 136 amino acids and predicts a molecular mass of 15.5 kDa. rhIL-9 migrates as multiple bands with the molecular mass of 18-25 kDa band in SDS-PAGE under reducing conditions due to different glycosylation.
Preparation: Baculovirus-Insect Cells
Purity: > 97 % as determined by SDS-PAGE
Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
Kit Component: N/A
Features & Benefits : N/A
Molecular Weight: 15.5 kDa
Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL9 protein, His Tag in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage Conditions: Lyophilized Human IL-9 protein, His Tagshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.69% HSA or BSA) . Please prevent freeze-thaw cycles.
Shipping: The product is shipped at ambient temperature.
Background: Interleukin 9, also known as IL-9, is a cytokine (cell signalling molecule) belonging to the group of interleukins. IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL-9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. IL-9 is a key molecule that affects differentiation of TH17 cells and Treg function. IL-9 predominantly produced by TH17 cells, synergizes with TGF-β1 to differentiate naïve CD4+ T cells into TH17 cells, while IL-9 secretion by TH17 cells is regulated by IL-23. Interestingly, IL-9 enhances the suppressive functions of FoxP3+ CD4+ Treg cells in vitro, and absence of IL-9 signaling weakens the suppressive activity of nTregs in vivo, leading to an increase in effector cells and worsening of experimental autoimmune encephalomyelitis. The mechanism of IL-9 effects on TH17 and Tregs is through activation of STAT3 and STAT5 signaling. Our findings highlight a role of IL-9 as a regulator of pathogenic versus protective mechanisms of immune responses.
Alternative Names: HP40; IL-9; P40; Interleukin-9; IL9
Search Names: HP40; IL-9; P40; Interleukin-9; IL9
Tag: IL-9
