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Product Description
Human TGF-β1 protein | PRP100190 | Abbkine
Product Category: Cytology
Application: Cytokines
Product Type: Cytokines
Sequence: Amino acid sequence derived from active form of human/rhesus/canine TGF-β1 (NP_000651.3) (Ala 279-Ser 390) was expressed and purified. Human, Rhesus and Canine TGF-β1 sequences are identical.
Activity: Measured by its ability to inhibit cell proliferation of Mv-1-lu mink lung epithelial cells. The ED50 for this effect is typically 0.04-0.2 ng/mL.
protein Lenght: The recombinant human/rhesus/canine TGF-β1 consists of 112 amino acids and has a calculated molecular mass of 12.8 kDa. it migrates as an approximately 13 & 26 kDa band in reduced and non-reduced SDS-PAGE respectively, corresponding to the monomer and homodimer.
Preparation: CHO Stable Cells
Purity: > 95 % as determined by HPLC.
Formulation: Lyophilized from sterile 100mM GLY, 10mM NaCl, pH 3.0
Kit Component: N/A
Features & Benefits : N/A
Molecular Weight: 12.8 kDa
Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human TGFB1 protein in 0.1% TFA, 26% ACN, PH3.0 not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage Conditions: Lyophilized Human TGF-β1 proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.57% HSA or BSA) . Please prevent freeze-thaw cycles.
Shipping: The product is shipped at ambient temperature.
Background: TGF-β1 is a member of the transforming growth factor β (TGF-β) family. The transforming growth factor-β family of polypeptides are involved in the regulation of cellular processes, including cell division, differentiation, motility, adhesion and death. TGF-β1 positively and negatively regulates many other growth factors. It inhibits the secretion and activity of many other cytokines including interferon-γ, tumor necrosis factor-α and various interleukins. It can also decrease the expression levels of cytokine receptors. Meanwhile, TGF-β1 also increases the expression of certain cytokines in T cells and promotes their proliferation, particularly if the cells are immature. TGF-β1 also inhibits proliferation and stimulates apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells. As for myeloid cells, TGF-β1can inhibit their proliferation and prevent their production of reactive oxygen and nitrogen intermediates. However, as with other cell types, TGF-β1 also has the opposite effect on cells of myeloid origin. TGF-β1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Once cells lose their sensitivity to TGF-β1-mediated growth inhibition, autocrine TGF-β signaling can promote tumorigenesis. Elevated levels of TGF-β1 are often observed in advanced carcinomas, and have been correlated with increased tumor invasiveness and disease progression.
Alternative Names: CED; DPD1; LAP; TGF-beta 1; TGFB; TGFbeta; Transforming growth factor beta-1; TGFB1
Search Names: CED; DPD1; LAP; TGF-beta 1; TGFB; TGFbeta; Transforming growth factor beta-1; TGFB1
Tag: TGF-β1
