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Product Description
CheKine™ Diamine Oxidase (DAO) Activity Colorimetric Assay Kit | KTB1220 | Abbkine
Product Category: Cytology
Application: Cell Metabolism
Product Type: Biochemical Assay Kit
Sequence: N/A
Activity: N/A
protein Lenght: N/A
Preparation: N/A
Purity: N/A
Formulation: N/A
Kit Component: • Extraction Buffer
• ReagentⅠ
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ
Features & Benefits : • simple, sensitive, rapid and colorimetric SDH activity detection method.
• compatible to various biological samples, especially animals or plant tissues or cell cultures (adherent or suspension), and fungi.
• Provides detailed sample preparation and results calculation methods.
Molecular Weight: N/A
Usage Note: • ΔA of Test Tube should be between 0.01-0.3, the sample should be diluted further if ΔA of Test Tube ＞0.3.
•Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
• Avoid bubbles while mixing or redissolving components.
• Change pipette tips frequently to avoid cross contamination between components.
• Ensure that all components and equipment are at the proper temperature before starting the experiment.
• All samples and reagents should be on ice to avoid denaturation and deactivation .
• Don't recommend to test too many samples in one time, because enzyme activity is calculated by the variation of the absorbance value per unit time .
• Recommended to calculate the enzyme activity by protein concentration. If you need to calculate by the sample weight, the total enzyme activity is equal to the enzyme activity of the supernatant and pellet which is cytoplasmic extraction.
• Please to extract SDH from fresh samples in order to ensure the enzyme activity .
• it is normal while there is fewer precipitates in Reagent Ⅲ solution. Please filter if the precipitates interfer the result .
• Please reduce the tissues weight if the OD values is too high . Or reduce the volume of Extraction Buffer if the OD values is too low .
Storage Conditions: The kit is valid for 6 months. Please refer to the table below Materials supplied and Storage conditions to store all the components.
Shipping: Gel pack with blue ice.
Background: Succinate dehydrogenase (SDH) is widely present in animals, plants, microorganisms and cultured cells. SDH is a marker enzyme of mitochondria, a film binding enzyme located on a mitochondrial inner membrane, and is one of the hubs connecting respiratory electron transfer and an oxidative phosphorylation. In addition, SDH can provide electron for the respiratory chain of a variety of prokaryotic cell capacity.
Alternative Names: N/A
Search Names: N/A
Tag: Succinate dehydrogenase，SDH
