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Product Description
CheKine™ Glucose-6-Phosphate Dehydrogenase (G6PDH) Activity Colorimetric Assay Kit | KTB1011 | Abbkine
Product Category: Cytology
Application: Cell Metabolism
Product Type: Biochemical Assay Kit
Sequence: N/A
Activity: N/A
protein Lenght: N/A
Preparation: N/A
Purity: N/A
Formulation: N/A
Kit Component: • Assay Buffer
• EtOH Solution
• WST-8 Solution
• Enhancer
• NAD Cycling Enzyme Mix
• NAD standard (10 mM)
• NAD Extraction Buffer
• NADH Extraction Buffer
Features & Benefits : • Detect NAD+/NADH concentrations and ratios in cell or tissue extracts.
• Detection limit of 0.78 µM and linearity up to 50 µM NAD+/NADH in 96-well plate assay.
Molecular Weight: N/A
Usage Note: • Addition of Working Reagent should be quick and mixing should be brief but thorough.
• The following substances interfere and should be avoided in sample preparation. EDTA (>0.5 mM), ascorbic acid, SDS (>0.2%), sodium azide, NP-40 (>1%) and Tween-20 (>1%) .
Storage Conditions: Storage at –20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping: Gel pack with blue ice.
Background: Nicotinamide adenine dinucleotide (NAD) is an enzymatic cofactor involved in many redox reactions. NAD functions as an electron carrier, cycling between the oxidized (NAD) and reduced (NADH) forms. In addition to its role in redox reactions, NAD plays critical roles in ADP-ribosylation reactions and as a substrate for sirtuins.
Alternative Names: N/A
Search Names: N/A
Tag: NAD/NADH;NAD;NADH
