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Product Description
CheKine™ Mitochondrial complexⅡ Activity Assay Kit (colorimetric) | KTB1860 | Abbkine
Product Category: Cytology
Application: Cell Metabolism
Product Type: Biochemical Assay Kit
Sequence: N/A
Activity: N/A
protein Lenght: N/A
Preparation: N/A
Purity: N/A
Formulation: N/A
Kit Component: • ReagentⅠ
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ
• Reagent Ⅴ
• Reagent Ⅵ
Features & Benefits : • Detect the enzyme activity of complex III in plant tissues, animal tissues, and cell samples.
• Provides detailed sample preparation and results calculation methods.
Molecular Weight: N/A
Usage Note: • Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
• Avoid bubbles while mixing or redissolving components.
• Change pipette tips frequently to avoid cross contamination between components.
• Ensure that all components and equipment are at the proper temperature before starting the experiment.
• In order to guarantee the accuracy of experimental results, need to do a pre-experiment with 1-2 samples. If the absorbance values is too high (above 1), the samples should be diluted with ReagentⅡand then measured again . Pay attention to multiply by the dilution factor when calculating the result.
• The protein concentration of the sample needs to be determined by users.
•Note that Reagent Ⅲ has certain toxicity, Please take protective measures during operation.
Storage Conditions: The kit is valid for 6 months. Please refer to the table below Materials supplied and Storage conditions to store all the components.
Shipping: Gel pack with blue ice.
Background: Mitochondrial respiratory chain complex III (EC 1.10.2.2), also known as CoQ-cytochrome C reductase, is widely present in the mitochondria of animals, plants, microorganisms and cultured cells. It plays a key role at the main and branch pathways in the mitochondrial respiratory electron transport chain. Complex III could transfer the hydrogen of the reduced CoQ to cytochrome C, and produce reduced cytochrome C.
Alternative Names: N/A
Search Names: N/A
Tag: complex Ⅲ
