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Product Description
Live and Dead Cell Double Staining Kit | KTA1001 | Abbkine
Product Category: Cytology
Application: Cellular states
Product Type: Apoptosis Detection Kit
Sequence: N/A
Activity: N/A
protein Lenght: N/A
Preparation: N/A
Purity: N/A
Formulation: N/A
Kit Component: • JC-1 Stain
• CCCP (10 mM)
• Assay Buffer (5×)
Features & Benefits : • Measure mitochondrial membrane potential as an indicator of cell health.
• Use JC-1, a fluorescent, lipophilic, cationic dye.
• Red fluorescence indicates healthy mitochondria and green fluorescence indicates mitochondria in poor health.
• Optimized for flow cytometery or fluorescent microscopy.
• Contain CCC (Potent mitochondrial oxidative phosphorylation uncoupler) .
Molecular Weight: N/A
Usage Note: • JC-1 Stain is difficult to prepare due to its low solubility. Make sure JC-1 Stain is completely thawed and warmed to room temperature before using.
• JC-1 Stain is light senstive. Incubations need to be done in the dark.
Storage Conditions: Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment.
Shipping: Gel pack with blue ice.
Background: Mitochondria, the site of most energy production in eukaryotic cells, have a double membrane structure: an outer membrane and a folded inner membrane. Across the inner membrane of intact mitochondria there is a voltage gradient with the inside negative and the outside positive. Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis.
Alternative Names: N/A
Search Names: N/A
Tag: JC-1
