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Product Description
Human Alpha-1-Acid Glycoprotein (A1AGP) ELISA Kit | KTE62558 | Abbkine
Application: This Human Alpha-1-Acid Glycoprotein (A1AGP) ELISA Kit employs a two-site sandwich ELISA to quantitate A1AGP in samples. An antibody specific for A1AGP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyA1AGP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for A1AGP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of A1AGP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Alpha-1-Acid Glycoprotein microplate
• Human Alpha-1-Acid Glycoprotein standard
• Human Alpha-1-Acid Glycoprotein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Alpha-1-Acid Glycoprotein (A1AGP) ELISA Kit has high sensitivity and excellent specificity for detection of Human A1AGP. No significant cross-reactivity or interference between Human A1AGP and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Alpha-1-acid glycoprotein (AGP) is a simplified name for orosomucoid (ORM) . ORM is an acute phase (Acute phase protein) plasma alpha-globulin glycoprotein and is modulated by two polymorphic genes. It is synthesized primarily in hepatocytes and has a normal plasma concentration between 0.6-1.2 mg/mL (1-3% plasma protein) .Plasma levels are affected by pregnancy, certain drugs, and certain diseases, particularly HIV. The only established function of ORM is to act as a carrier of basic and neutrally charged lipophilic compounds. In medicine, it is known as the primary carrier of basic drugs (whereas albumin carries acidic drugs), steroids, and protease inhibitors.Orosomucoid increases in amount in obstructive jaundices while diminishes in hepatocellular jaundice and in intestinal infections.
Alternative Names: A1AGP; AGP1; a1-AGP; ORM1; Orosomucoid 1
Search name: A1AGP; AGP1; a1-AGP; ORM1; Orosomucoid 1
Tag: A1AGP
