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Product Description
Human Alpha-1-microglobulin/bikunin precursor (AMBP) ELISA Kit | KTE62304 | Abbkine
Application: This Human Alpha-1-microglobulin/bikunin precursor (AMBP) ELISA Kit employs a two-site sandwich ELISA to quantitate AMBP in samples. An antibody specific for AMBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAMBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMBP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Alpha-1-microglobulin/bikunin precursor microplate
• Human Alpha-1-microglobulin/bikunin precursor standard
• Human Alpha-1-microglobulin/bikunin precursor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Alpha-1-microglobulin/bikunin precursor (AMBP) ELISA Kit has high sensitivity and excellent specificity for detection of Human AMBP. No significant cross-reactivity or interference between Human AMBP and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Alpha-1-microglobulin (A1M), also known as protein HC (for Heterogeneous Charge), is a low molecular weight protein component of plasma first discovered in pathological human urine. It is a member of the lipocalin superfamily. Although much is now known of its structure and properties, the function and physiological role of A1M remains unclear, although evidence suggests that it functions in the regulation of the immune system. A1M is known to exist in both a free form and complexed to other macromolecules: immunoglobulin A (IgA) in humans and alpha-1-inhibitor-3 in the rat. Free A1M is a monomeric protein composed of one 188 residue polypeptide and contains three cysteines, two of which (residues 75 and 173) form a conserved intra-molecular disulphide link.
Alternative Names: AMBP; A1M; EDC1; HCP; HI30; IATIL; ITI; ITIL; ITILC; UTI; alpha-1-microglobulin/bikunin; bikunin; complex-forming glycoprotein heterogeneous in charge; growth-inhibiting protein 19; inter-alpha-trypsin inhibi
Search name: AMBP; A1M; EDC1; HCP; HI30; IATIL; ITI; ITIL; ITILC; UTI; alpha-1-microglobulin/bikunin; bikunin; complex-forming glycoprotein heterogeneous in charge; growth-inhibiting protein 19; inter-alpha-trypsin inhibi
Tag: AMBP
