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Product Description
Human Anti-Luteinizing Hormone Antibody (Anti-LH-Ab) ELISA Kit | KTE62458 | Abbkine
Application: This Human Anti-Luteinizing Hormone Antibody (Anti-LH-Ab) ELISA Kit employs a two-site sandwich ELISA to quantitate Anti-LH-Ab in samples. An antibody specific for Anti-LH-Ab has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAnti-LH-Ab present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Anti-LH-Ab is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-LH-Ab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Anti-Luteinizing Hormone Antibody microplate
• Human Anti-Luteinizing Hormone Antibody standard
• Human Anti-Luteinizing Hormone Antibody detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Anti-Luteinizing Hormone Antibody (Anti-LH-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Human Anti-LH-Ab. No significant cross-reactivity or interference between Human Anti-LH-Ab and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Luteinizing hormone (LH) is an important hormone of the reproductive system, which has found application in diagnosis and therapeutic medicine. It plays a vital role in the development and functioning of the reproductive system. Determination of LH concentration is important for detection of dysfunction of the pituitary variance axis, diagnosis of reproductive disorders, monitoring of antifertility programmes and in therapeutic preparations.LH levels are normally low during childhood and, in women, high after menopause. As LH is secreted as pulses, it is necessary to follow its concentration over a sufficient period of time to get a proper information about its blood level.During the reproductive years typical levels are between 1-20 IU/L. Physiologic high LH levels are seen during the LH surge (v.s.) ; typically they last 48 hours.
Alternative Names: Anti-LH-Ab
Search name: Anti-LH-Ab
Tag: Anti-LH
