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Product Description
Human ATP-dependent RNA helicase A (DHX9) ELISA Kit | KTE62062 | Abbkine
Application: This Human ATP-dependent RNA helicase A (DHX9) ELISA Kit employs a two-site sandwich ELISA to quantitate DHX9 in samples. An antibody specific for DHX9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDHX9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHX9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHX9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human ATP-dependent RNA helicase A microplate
• Human ATP-dependent RNA helicase A standard
• Human ATP-dependent RNA helicase A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human ATP-dependent RNA helicase A (DHX9) ELISA Kit has high sensitivity and excellent specificity for detection of Human DHX9. No significant cross-reactivity or interference between Human DHX9 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: DHX9 encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes to both the nucleus and the cytoplasm and functions as a transcriptional regulator. This protein may also be involved in the expression and nuclear export of retroviral RNAs. Alternate splicing results in multiple transcript variants. Pseudogenes of DHX9 are found on chromosomes 11 and 13.
Alternative Names: DHX9; DDX9; LKP; NDHII; RHA; ATP-dependent RNA helicase A; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9 (RNA helicase A) ; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9 (RNA helicase A; nuclear DNA helica
Search name: DHX9; DDX9; LKP; NDHII; RHA; ATP-dependent RNA helicase A; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9 (RNA helicase A) ; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9 (RNA helicase A; nuclear DNA helica
Tag: DHX9
