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Product Description
Human Baculoviral IAP repeat-containing protein 4 (XIAP) ELISA Kit | KTE62596 | Abbkine
Application: This Human Baculoviral IAP repeat-containing protein 4 (XIAP) ELISA Kit employs a two-site sandwich ELISA to quantitate XIAP in samples. An antibody specific for XIAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXIAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XIAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XIAP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Baculoviral IAP repeat-containing protein 4 microplate
• Human Baculoviral IAP repeat-containing protein 4 standard
• Human Baculoviral IAP repeat-containing protein 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Baculoviral IAP repeat-containing protein 4 (XIAP) ELISA Kit has high sensitivity and excellent specificity for detection of Human XIAP. No significant cross-reactivity or interference between Human XIAP and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: X-linked Inhibitor of Apoptosis Protein (XIAP) is a member of the Inhibitor of apoptosis family of proteins (IAP) . IAPs were initially identified in baculoviruses, but XIAP is one of the homologous proteins found in mammals. It is so called because it was first discovered by a 273 base pair site on the X chromosome.The protein is also called human IAP-like Protein (hILP), because it is not as well conserved as the human IAPS: hIAP-1 and hIAP-2. XIAP is the most potent human IAP protein currently identified.XIAP, like the rest of the IAP family, has two major structural elements. Firstly, there is the baculoviral IAP repeat (BIR) domain consisting of approximately 70 amino acids. Secondly, there is a zinc-binding domain, or a “carboxy-terminal RING Finger”.
Alternative Names: XIAP; RP1-315G1.5; API3; BIRC4; ILP1; MIHA; XLP2; OTTHUMP00000196392; apoptosis inhibitor 3; baculoviral IAP repeat-containing 4; baculoviral IAP repeat-containing protein 4
Search name: XIAP; RP1-315G1.5; API3; BIRC4; ILP1; MIHA; XLP2; OTTHUMP00000196392; apoptosis inhibitor 3; baculoviral IAP repeat-containing 4; baculoviral IAP repeat-containing protein 4
Tag: XIAP
