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Product Description
Human Breast cancer susceptibility protein 1 (BRCA1) ELISA Kit | KTE62651 | Abbkine
Application: This Human Breast cancer susceptibility protein 1 (BRCA1) ELISA Kit employs a two-site sandwich ELISA to quantitate BRCA1 in samples. An antibody specific for BRCA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBRCA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BRCA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BRCA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Breast cancer susceptibility protein 1 microplate
• Human Breast cancer susceptibility protein 1 standard
• Human Breast cancer susceptibility protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Breast cancer susceptibility protein 1 (BRCA1) ELISA Kit has high sensitivity and excellent specificity for detection of Human BRCA1. No significant cross-reactivity or interference between Human BRCA1 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: BRCA1 is a human gene, some mutations of which are associated with a significant increase in the risk of breast cancer, as well as other cancers. BRCA1 belongs to a class of genes known as tumor suppressors, which maintains genomic integrity to prevent dangerous genetic changes. The multifactorial BRCA1 protein product is involved in DNA damage repair especially error-free repair of DNA double strand breaks, ubiquitination, transcriptional regulation as well as other functions.The BRCA1 protein is directly involved in the repair of damaged DNA. In the nucleus of many types of normal cells, the BRCA1 protein is thought to interact indirectly with RAD51 during repair of DNA double-strand breaks, though the details and significance of this interaction is the subject of debate.
Alternative Names: BRCA1; BRCAI; BRCC1; BROVCA1; IRIS; PSCP; RNF53; BRCA1/BRCA2-containing complex; subunit 1; RING finger protein 53; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibili
Search name: BRCA1; BRCAI; BRCC1; BROVCA1; IRIS; PSCP; RNF53; BRCA1/BRCA2-containing complex; subunit 1; RING finger protein 53; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibili
Tag: BRCA1
