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Product Description
Human Chordin (CHRD) ELISA Kit | KTE62283 | Abbkine
Application: This Human Chordin (CHRD) ELISA Kit employs a two-site sandwich ELISA to quantitate CHRD in samples. An antibody specific for CHRD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCHRD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Chordin microplate
• Human Chordin standard
• Human Chordin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Chordin (CHRD) ELISA Kit has high sensitivity and excellent specificity for detection of Human CHRD. No significant cross-reactivity or interference between Human CHRD and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Chordin is a polypeptide that dorsalizes the developing embryo by binding ventralizing TGFβ proteins such as bone morphogenetic proteins. It may also play a role in organogenesis. There are five named isoforms of this protein that are produced by alternative splicing.In humans, the chordin peptide is encoded by the CHRD gene. 948-amino acid protein containing 4 internal cysteine-rich repeats and 4 potential N-glycosylation sites. Northern blot analysis detected high levels of a 3.7-kb Chrd transcript in 7-day postcoitum mouse embryos; its level decreased at later developmental stages and in adult tissues. Dot blot analysis of mRNA expression in human adult and fetal tissues showed that CHRD is expressed at the highest level in liver.
Alternative Names: CHRD; MGC133038;
Search name: CHRD; MGC133038;
Tag: CHRD
