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Product Description
Human Cullin-9 (CUL9) ELISA Kit | KTE62415 | Abbkine
Application: This Human Cullin-9 (CUL9) ELISA Kit employs a two-site sandwich ELISA to quantitate CUL9 in samples. An antibody specific for CUL9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCUL9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CUL9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CUL9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Cullin-9 microplate
• Human Cullin-9 standard
• Human Cullin-9 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Cullin-9 (CUL9) ELISA Kit has high sensitivity and excellent specificity for detection of Human CUL9. No significant cross-reactivity or interference between Human CUL9 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: PARC directly interacted and formed an approximately 1-MD complex with p53 in the cytoplasm of unstressed cells. In the absence of stress, inactivation of PARC induced nuclear localization of endogenous p53 and activated p53-dependent apoptosis. Overexpression of PARC promoted cytoplasmic sequestration of ectopic p53.abnormal cytoplasmic localization of p53 was observed in a number of neuroblastoma cell lines; RNA interference-mediated reduction of endogenous PARC significantly sensitized these neuroblastoma cells in the DNA damage response. These results revealed that PARC is a critical regulator in controlling p53 subcellular localization and subsequent function.PARC also has a unique motif that is highly homologous to the C terminus of cullin proteins that the authors referred to as the C-terminal cullin homology domain.
Alternative Names: CUL9; DKFZp686G1042; DKFZp686P2024; H7AP1; PARC; RP3-330M21.2; UbcH7-associated protein 1; p53-associated parkin-like cytoplasmic protein; parkin-like cytoplasmic p53 binding protein
Search name: CUL9; DKFZp686G1042; DKFZp686P2024; H7AP1; PARC; RP3-330M21.2; UbcH7-associated protein 1; p53-associated parkin-like cytoplasmic protein; parkin-like cytoplasmic p53 binding protein
Tag: CUL9
