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Product Description
Human Cyclin-D3 (CCND3) ELISA Kit | KTE62200 | Abbkine
Application: This Human Cyclin-D3 (CCND3) ELISA Kit employs a two-site sandwich ELISA to quantitate CCND3 in samples. An antibody specific for CCND3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCCND3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCND3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCND3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Cyclin-D3 microplate
• Human Cyclin-D3 standard
• Human Cyclin-D3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Cyclin-D3 (CCND3) ELISA Kit has high sensitivity and excellent specificity for detection of Human CCND3. No significant cross-reactivity or interference between Human CCND3 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: The protein encoded by CCND3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for CCND3.
Alternative Names: CCND3; RP5-973N23.3; D3-type cyclin; G1/S-specific cyclin D3
Search name: CCND3; RP5-973N23.3; D3-type cyclin; G1/S-specific cyclin D3
Tag: CCND3
