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Product Description
Human Cytochrome P450 3A4 (CYP3A4) ELISA Kit | KTE62160 | Abbkine
Application: This Human Cytochrome P450 3A4 (CYP3A4) ELISA Kit employs a two-site sandwich ELISA to quantitate CYP3A4 in samples. An antibody specific for CYP3A4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYP3A4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP3A4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP3A4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Cytochrome P450 3A4 microplate
• Human Cytochrome P450 3A4 standard
• Human Cytochrome P450 3A4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Cytochrome P450 3A4 (CYP3A4) ELISA Kit has high sensitivity and excellent specificity for detection of Human CYP3A4. No significant cross-reactivity or interference between Human CYP3A4 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: CYP3A4 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. CYP3A4 is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified.
Alternative Names: CYP3A4; CP33; CP34; CYP3A; CYP3A3; HLP; MGC126680; NF-25; P450C3; P450PCN1; P450-III; steroid inducible; cytochrome P450; subfamilyIA (niphedipine oxidase) ; polypeptide 3; cytochrome P450; subfamilyIA (
Search name: CYP3A4; CP33; CP34; CYP3A; CYP3A3; HLP; MGC126680; NF-25; P450C3; P450PCN1; P450-III; steroid inducible; cytochrome P450; subfamilyIA (niphedipine oxidase) ; polypeptide 3; cytochrome P450; subfamilyIA (
Tag: CYP3A4
