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Product Description
Human Deleted in malignant brain tumors 1 protein (DMBT1) ELISA Kit | KTE62015 | Abbkine
Application: This Human Deleted in malignant brain tumors 1 protein (DMBT1) ELISA Kit employs a two-site sandwich ELISA to quantitate DMBT1 in samples. An antibody specific for DMBT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMBT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMBT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMBT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Deleted in malignant brain tumors 1 protein microplate
• Human Deleted in malignant brain tumors 1 protein standard
• Human Deleted in malignant brain tumors 1 protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Deleted in malignant brain tumors 1 protein (DMBT1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DMBT1. No significant cross-reactivity or interference between Human DMBT1 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Loss of sequences from human chromosome 10q has been associated with the progression of human cancers. The gene DMBT1 was originally isolated based on its deletion in a medulloblastoma cell line. DMBT1 is expressed with transcripts of 6.0, 7.5, and 8.0 kb in fetal lung and with one transcript of 8.0 kb in adult lung, although the 7.5 kb transcript has not been characterized. The DMBT1 protein is a glycoprotein containing multiple scavenger receptor cysteine-rich (SRCR) domains separated by SRCR-interspersed domains (SID) . Transcript variant 2 (8.0 kb) has been shown to bind surfactant protein D independently of carbohydrate recognition. This indicates that DMBT1 may not be a classical tumor supressor gene, but rather play a role in the interaction of tumor cells and the immune system.
Alternative Names: DMBT1; RP11-481L19.1; FLJ61058; GP340; MGC164738; muclin;
Search name: DMBT1; RP11-481L19.1; FLJ61058; GP340; MGC164738; muclin;
Tag: DMBT1
