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Product Description
Human DEP domain-containing protein 1B (DEPDC1B) ELISA Kit | KTE62096 | Abbkine
Application: This Human DEP domain-containing protein 1B (DEPDC1B) ELISA Kit employs a two-site sandwich ELISA to quantitate DEPDC1B in samples. An antibody specific for DEPDC1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDEPDC1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEPDC1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEPDC1B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human DEP domain-containing protein 1B microplate
• Human DEP domain-containing protein 1B standard
• Human DEP domain-containing protein 1B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human DEP domain-containing protein 1B (DEPDC1B) ELISA Kit has high sensitivity and excellent specificity for detection of Human DEPDC1B. No significant cross-reactivity or interference between Human DEPDC1B and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: DEP Domain Containing Protein 1B also known as XTP1, XTP8, HBV XAg-Transactivated Protein 8, [formerly referred to as BRCC3] is a human protein encoded by a gene of similar name located on chromosome 5. The precise function of DEPDC1B is currently unknown. Expression profiles indicate that DEPDC1B is highly expressed ubiquitously throughout human tissue.
Alternative Names: DEPDC1B; BRCC3; FLJ11252; XTP1; HBxAg transactivated protein 1; breast cancer cell 3
Search name: DEPDC1B; BRCC3; FLJ11252; XTP1; HBxAg transactivated protein 1; breast cancer cell 3
Tag: DEPDC1B
