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Product Description
Human Diablo homolog, mitochondrial (DIABLO) ELISA Kit | KTE62061 | Abbkine
Application: This Human Diablo homolog, mitochondrial (DIABLO) ELISA Kit employs a two-site sandwich ELISA to quantitate DIABLO in samples. An antibody specific for DIABLO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDIABLO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DIABLO is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DIABLO bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Diablo homolog, mitochondrial microplate
• Human Diablo homolog, mitochondrial standard
• Human Diablo homolog, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Diablo homolog, mitochondrial (DIABLO) ELISA Kit has high sensitivity and excellent specificity for detection of Human DIABLO. No significant cross-reactivity or interference between Human DIABLO and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: DIABLO encodes an inhibitor of apoptosis protein (IAP) -binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in DIABLO is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms.
Alternative Names: DIABLO; hCG_1782202; DIABLO-S; FLJ10537; FLJ25049; SMAC; SMAC3; 0610041G12Rik; diablo; direct IAP-binding protein with low pI; mitochondrial Smac protein; second mitochondria-derived activator of caspase
Search name: DIABLO; hCG_1782202; DIABLO-S; FLJ10537; FLJ25049; SMAC; SMAC3; 0610041G12Rik; diablo; direct IAP-binding protein with low pI; mitochondrial Smac protein; second mitochondria-derived activator of caspase
Tag: DIABLO
