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Product Description
Human EGF-like repeat and discoidin I-like domain-containing protein 3 (EDIL3) ELISA Kit | KTE62404 | Abbkine
Application: This Human EGF-like repeat and discoidin I-like domain-containing protein 3 (EDIL3) ELISA Kit employs a two-site sandwich ELISA to quantitate EDIL3 in samples. An antibody specific for EDIL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEDIL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EDIL3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EDIL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human EGF-like repeat and discoidin I-like domain-containing protein 3 microplate
• Human EGF-like repeat and discoidin I-like domain-containing protein 3 standard
• Human EGF-like repeat and discoidin I-like domain-containing protein 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human EGF-like repeat and discoidin I-like domain-containing protein 3 (EDIL3) ELISA Kit has high sensitivity and excellent specificity for detection of Human EDIL3. No significant cross-reactivity or interference between Human EDIL3 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: EDIL3 is a glycoprotein that is secreted by endothelial cells and can associate with the endothelial cell surface and the extracellular matrix. EDIL3 is regulated by hypoxia or vascular injury and has been implicated in vascular remodeling during angiogenesis.The predicted 480-amino acid mouse and human proteins, which are 95% identical, contain a signal peptide, 3 epidermal growth factor (EGF) -like repeats, and 2 discoidin I-like repeats. Northern blot analysis revealed expression of a 6.5-kb transcript in highly vascularized mouse embryonic day-15.5 tissue and in mouse and human endothelial cells, but not in other adult tissues. In situ hybridization analysis showed Del1 expression in developing endocardium at 9.5 days postcoitus and in renal artery and hypertrophic chondrocytes at 13.5 days postcoitus.
Alternative Names: EDIL3; DEL1; MGC26287; EGF-like repeats and discoidin I-like domains-containing protein 3; developmental endothelial locus-1
Search name: EDIL3; DEL1; MGC26287; EGF-like repeats and discoidin I-like domains-containing protein 3; developmental endothelial locus-1
Tag: EDIL3
