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Product Description
Human Endothelial cell-specific molecule 1 (ESM-1) ELISA Kit | KTE62398 | Abbkine
Application: This Human Endothelial cell-specific molecule 1 (ESM-1) ELISA Kit employs a two-site sandwich ELISA to quantitate ESM1 in samples. An antibody specific for ESM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyESM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ESM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ESM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Endothelial cell-specific molecule 1 microplate
• Human Endothelial cell-specific molecule 1 standard
• Human Endothelial cell-specific molecule 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Endothelial cell-specific molecule 1 (ESM-1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ESM1. No significant cross-reactivity or interference between Human ESM1 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Endothelial cell-specific molecule 1 is encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. The cDNA sequence contains an open reading frame of 552 nucleotides. The deduced 184-amino acid protein is cysteine rich with an N-terminal hydrophobic signal sequence. Expression of ESM1 was tested by Northern blot analysis and RT-PCR and was found to be constitutive in a HUVEC cell line, but not in any other cell line tested. Constitutive ESM1 expression in human tissue is restricted to the lung. Immunoblotting and immunoprecipitation identified a 20-kD protein.
Alternative Names: ESM1; endocan;
Search name: ESM1; endocan;
Tag: ESM1
