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Product Description
Human Epithelial-stromal interaction protein 1 (EPSTI1) ELISA Kit | KTE62401 | Abbkine
Application: This Human Epithelial-stromal interaction protein 1 (EPSTI1) ELISA Kit employs a two-site sandwich ELISA to quantitate EPSTI1 in samples. An antibody specific for EPSTI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEPSTI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EPSTI1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPSTI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Epithelial-stromal interaction protein 1 microplate
• Human Epithelial-stromal interaction protein 1 standard
• Human Epithelial-stromal interaction protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Epithelial-stromal interaction protein 1 (EPSTI1) ELISA Kit has high sensitivity and excellent specificity for detection of Human EPSTI1. No significant cross-reactivity or interference between Human EPSTI1 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: By differential display, Nielsen et al. (2002) identified EPSTI1 as a transcript that is induced by coculture of breast tumor cells and fibroblasts, and they generated the full-length cDNA by 5-prime RACE of normal breast or placenta RNA. The deduced 307-amino acid protein has a calculated molecular mass of about 35 kD. It contains no N-terminal signal sequence and no transmembrane domains; however, there are 3 coiled-coil regions. Northern blot analysis revealed a transcript size of 1.5 kb. Real-time PCR revealed highest expression in placenta; strong expression in small intestine, spleen, salivary gland, and testes; and low levels of expression in normal breast and several other tissues. The expression of EPSTI1 was upregulated up to 72 times (range 5.6-72.1) in all 14 breast carcinomas tested.
Alternative Names: EPSTI1; BRESI1; MGC29634; epithelial stromal interaction 1
Search name: EPSTI1; BRESI1; MGC29634; epithelial stromal interaction 1
Tag: EPSTI1
