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Product Description
Human Fibroblast growth factor 9 (glia-activating factor) (FGF9) ELISA Kit | KTE62587 | Abbkine
Application: This Human Fibroblast growth factor 9 (glia-activating factor) (FGF9) ELISA Kit employs a two-site sandwich ELISA to quantitate FGF9 in samples. An antibody specific for FGF9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFGF9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Fibroblast growth factor 9 microplate
• Human Fibroblast growth factor 9 standard
• Human Fibroblast growth factor 9 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Fibroblast growth factor 9 (glia-activating factor) (FGF9) ELISA Kit has high sensitivity and excellent specificity for detection of Human FGF9. No significant cross-reactivity or interference between Human FGF9 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Fibroblast growth factor 9 (FGF9) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis.
Alternative Names: FGF9; GAF; HBFG-9; MGC119914; MGC119915; SYNS3; fibroblast growth factor 9
Search name: FGF9; GAF; HBFG-9; MGC119914; MGC119915; SYNS3; fibroblast growth factor 9
Tag: FGF9
