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Product Description
Human Folate receptor beta (FOLR2) ELISA Kit | KTE62254 | Abbkine
Application: This Human Folate receptor beta (FOLR2) ELISA Kit employs a two-site sandwich ELISA to quantitate FOLR2 in samples. An antibody specific for FOLR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFOLR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FOLR2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FOLR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Folate receptor beta microplate
• Human Folate receptor beta standard
• Human Folate receptor beta detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Folate receptor beta (FOLR2) ELISA Kit has high sensitivity and excellent specificity for detection of Human FOLR2. No significant cross-reactivity or interference between Human FOLR2 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Folate receptor beta is a member of the folate receptor (FOLR) family, and these genes exist in a cluster on chromosome 11. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and they mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This protein has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. Multiple transcript variants that encode the same protein have been found for this gene.
Alternative Names: FOLR2; BETA-HFR; FBP/PL-1; FR-BETA; FR-P3; folate receptor 2; folate receptor; beta; folate-binding protein; fetal/placental
Search name: FOLR2; BETA-HFR; FBP/PL-1; FR-BETA; FR-P3; folate receptor 2; folate receptor; beta; folate-binding protein; fetal/placental
Tag: FOLR2
