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Product Description
Human Galectin-1 (LGALS1) ELISA Kit | KTE61825 | Abbkine
Application: This Human Galectin-1 (LGALS1) ELISA Kit employs a two-site sandwich ELISA to quantitate LGALS1 in samples. An antibody specific for LGALS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGALS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGALS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Galectin-1 microplate
• Human Galectin-1 standard
• Human Galectin-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Galectin-1 (LGALS1) ELISA Kit has high sensitivity and excellent specificity for detection of Human LGALS1. No significant cross-reactivity or interference between Human LGALS1 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Galectin-1 is one of the most important lectins to date participating in the malignant tumor development. With more and more data available on its expression in tumors it is already clear that this lectin is an important target for cancer diagnostics and treatment.Its first involvement in cancer was associated with cell adhesion. Later on the lectin was shown to induce apoptosis of activated T-cells, and experiments have confirmed that cancer cells express galectin-1 also to protect themselves from immune response. Latest finding is a participation of galectin-1 in tumor angiogenesis as an angiogenic factor. In most of the cases described, galectin-0 is up-regulated in tumors in comparison to normal tissue. Moreover, the presence of the lectin is associated with metastases and poor prognosis of disease outcome.
Alternative Names: LGALS1; DKFZp686E23103; GAL1; GBP; S-Lac lectin 1; beta-galactoside-binding lectin L-14-I; galaptin; galectin 1; galectin-1; lactose-binding lectin 1
Search name: LGALS1; DKFZp686E23103; GAL1; GBP; S-Lac lectin 1; beta-galactoside-binding lectin L-14-I; galaptin; galectin 1; galectin-1; lactose-binding lectin 1
Tag: LGALS1
