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Product Description
Human Interferon alpha-21 (IFNA21) ELISA Kit | KTE62246 | Abbkine
Application: This Human Interferon alpha-21 (IFNA21) ELISA Kit employs a two-site sandwich ELISA to quantitate IFNA21 in samples. An antibody specific for IFNA21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIFNA21 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFNA21 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFNA21 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Interferon alpha-21 microplate
• Human Interferon alpha-21 standard
• Human Interferon alpha-21 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Interferon alpha-21 (IFNA21) ELISA Kit has high sensitivity and excellent specificity for detection of Human IFNA21. No significant cross-reactivity or interference between Human IFNA21 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: The IFNA21 gene was positioned in the cluster of interferon genes on 9p22 by deletion mapping. Interferons (IFNs) are proteins made and released by lymphocytes in response to the presence of pathogens—such as viruses, bacteria, or parasites—or tumor cells. IFNs belong to the large class of glycoproteins known as cytokines. Although they are named after their ability to "interfere" with viral replication within host cells, IFNs have other functions: they activate immune cells, such as natural killer cells and macrophages; they increase recognition of infection or tumor cells by up-regulating antigen presentation to T lymphocytes; and they increase the ability of uninfected host cells to resist new infection by virus. Certain host symptoms, such as aching muscles and fever, are related to the production of IFNs during infection.
Alternative Names: IFNA21; MGC126687; MGC126689; leukocyte interferon protein
Search name: IFNA21; MGC126687; MGC126689; leukocyte interferon protein
Tag: IFNA21
