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Product Description
Human Interleukin-10 receptor subunit beta (IL10RB) ELISA Kit | KTE61931 | Abbkine
Application: This Human Interleukin-10 receptor subunit beta (IL10RB) ELISA Kit employs a two-site sandwich ELISA to quantitate IL10RB in samples. An antibody specific for IL10RB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL10RB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL10RB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL10RB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Interleukin-10 receptor subunit beta microplate
• Human Interleukin-10 receptor subunit beta standard
• Human Interleukin-10 receptor subunit beta detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Interleukin-10 receptor subunit beta (IL10RB) ELISA Kit has high sensitivity and excellent specificity for detection of Human IL10RB. No significant cross-reactivity or interference between Human IL10RB and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Interleukin-10 receptor subunit beta encoded by IL10RB belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. IL10RB and three other interferon receptor genes, IFAR2, IFNAR1, and IFNGR2, form a class II cytokine receptor gene cluster located in a small region on chromosome 21.
Alternative Names: IL10RB; CDW210B; CRF2-4; CRFB4; D21S58; D21S66; IL-10R2; cytokine receptor class-II CRF2-4; cytokine receptor family; member 4
Search name: IL10RB; CDW210B; CRF2-4; CRFB4; D21S58; D21S66; IL-10R2; cytokine receptor class-II CRF2-4; cytokine receptor family; member 4
Tag: IL10RB
