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Product Description
Human Kallikrein 3 (KLK3) ELISA Kit | KTE62726 | Abbkine
Application: This Human Kallikrein 3 (KLK3) ELISA Kit employs a two-site sandwich ELISA to quantitate KLK3 in samples. An antibody specific for KLK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Kallikrein 3 microplate
• Human Kallikrein 3 standard
• Human Kallikrein 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Kallikrein 3 (KLK3) ELISA Kit has high sensitivity and excellent specificity for detection of Human KLK3. No significant cross-reactivity or interference between Human KLK3 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Human prostate-specific antigen (APS) is a kallikrein-like protease present in seminal plasma. It is a single-chain glycoprotein with a molecular mass of about 33 kD that may function normally in the liquefaction of seminal coagulum. Radioimmunoassay of serum levels of this antigen (called PSA in the clinical setting) is useful in the diagnosis and monitoring of prostatic carcinoma. The full-length KLK3 protein contains 261 amino acids. It has a putative signal peptide, followed by a short activating peptide and the protease domain, which includes the catalytic triad of his65, asp120, and ser213. RT-PCR of 35 adult and fetal tissues detected highest expression in prostate. Expression was weak in colon, mammary gland, and parotid, and little to no expression was detected in other tissues examined.
Alternative Names: KLK3; APS; KLK2A1; PSA; hK3; P-30 antigen; gamma-seminoprotein; prostate specific antigen; semenogelase; seminin
Search name: KLK3; APS; KLK2A1; PSA; hK3; P-30 antigen; gamma-seminoprotein; prostate specific antigen; semenogelase; seminin
Tag: KLK3
