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Product Description
Human Kelch-like ECH-associated protein 1 (KEAP1) ELISA Kit | KTE61924 | Abbkine
Application: This Human Kelch-like ECH-associated protein 1 (KEAP1) ELISA Kit employs a two-site sandwich ELISA to quantitate KEAP1 in samples. An antibody specific for KEAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKEAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KEAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KEAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Kelch-like ECH-associated protein 1 microplate
• Human Kelch-like ECH-associated protein 1 standard
• Human Kelch-like ECH-associated protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Kelch-like ECH-associated protein 1 (KEAP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human KEAP1. No significant cross-reactivity or interference between Human KEAP1 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: KEAP1 encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.Kelch motifs of Keap1 interact with the Neh2 domain of Nrf2 and that this interaction is required for the repression of Nrf2 by Keap1. Fluorescence microscopy demonstrated that Keap1 is expressed in the cytoplasm with Nrf2.
Alternative Names: KEAP1; INrf2; KIAA0132; KLHL19; MGC10630; MGC1114; MGC20887; MGC4407; MGC9454; cytosolic inhibitor of Nrf2
Search name: KEAP1; INrf2; KIAA0132; KLHL19; MGC10630; MGC1114; MGC20887; MGC4407; MGC9454; cytosolic inhibitor of Nrf2
Tag: KEAP1
