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Product Description
Human Left-right determination factor 2 (LEFTY2) ELISA Kit | KTE62691 | Abbkine
Application: This Human Left-right determination factor 2 (LEFTY2) ELISA Kit employs a two-site sandwich ELISA to quantitate LEFTY2 in samples. An antibody specific for LEFTY2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLEFTY2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LEFTY2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LEFTY2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Left-right determination factor 2 microplate
• Human Left-right determination factor 2 standard
• Human Left-right determination factor 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Left-right determination factor 2 (LEFTY2) ELISA Kit has high sensitivity and excellent specificity for detection of Human LEFTY2. No significant cross-reactivity or interference between Human LEFTY2 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Lefty proteins are signaling ligands that act in opposition to the NODAL protein. During vertebrate development they regulate the degree of left-right asymmetry by controlling the spatiotemporal influence of the NODAL protein.It is involved in embryogenesis and left-right patterning. There are many differences between the left and right sides, including heart and lung positioning. Mutations in these genes cause incorrect positioning of these organs (e.g., situs inversus) . Lefty1 in the ventral midline prevents the Cerebrus (paracrine factor or "Caronte") signal from passing to the right side of the embryo.The role of Lefty1 is to restrict the expression of Lefty2 and Nodal to the left side, and that Lefty2 or Nodal encode a signal for 'leftness.' Lefty2 serves as a feedback inhibitor to restrict the range of nodal signaling during establishment of the left-right axis.
Alternative Names: LEFTY2; EBAF; LEFTA; LEFTYA; MGC46222; TGFB4; endometrial bleeding associated factor; endometrial bleeding associated factor (left-right determination; factor A; transforming growth factor beta superfamily) ;
Search name: LEFTY2; EBAF; LEFTA; LEFTYA; MGC46222; TGFB4; endometrial bleeding associated factor; endometrial bleeding associated factor (left-right determination; factor A; transforming growth factor beta superfamily) ;
Tag: LEFTY2
