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Product Description
Human Lipoprotein α (Lp-a) ELISA Kit | KTE61786 | Abbkine
Application: This Human Lipoprotein α (Lp-a) ELISA Kit employs a two-site sandwich ELISA to quantitate Lp-a in samples. An antibody specific for Lp-a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLp-a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Lp-a is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Lp-a bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Lipoprotein α microplate
• Human Lipoprotein α standard
• Human Lipoprotein α detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Lipoprotein α (Lp-a) ELISA Kit has high sensitivity and excellent specificity for detection of Human Lp-a. No significant cross-reactivity or interference between Human Lp-a and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Lipoprotein (a) consists of an LDL-like particle and the specific apolipoprotein (a), which is covalently bound to the apoB of the LDL like particle. Lp (a) plasma concentrations are highly heritable and mainly controlled by the apolipoprotein (a) gene located on chromosome 6q26-27. Apo (a) proteins vary in size due to a size polymorphism, which is caused by a variable number of so called kringle IV repeats in the LPA gene. This size variation at the gene level is expressed on the protein level as well, resulting in apo (a) proteins with 10 to > 50 kringle IV repeats . These variable apo (a) sizes are known as "apo (a) isoforms". There is a general inverse correlation between the size of the apo (a) isoform and the Lp (a) plasma concentration which is caused by a variable rate of degradation before the apo (a) protein has matured for Lp (a) assembly.
Alternative Names: Lp-a; LP-A; AK38; APOA; LP; OTTHUMP00000017543; antiangiogenic AK38 protein; apolipoprotein (a) ; lipoprotein Lp (a)
Search name: Lp-a; LP-A; AK38; APOA; LP; OTTHUMP00000017543; antiangiogenic AK38 protein; apolipoprotein (a) ; lipoprotein Lp (a)
Tag: Lp-a
