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Product Description
Human Methionine synthase reductase (MTRR) ELISA Kit | KTE61474 | Abbkine
Application: This Human Methionine synthase reductase (MTRR) ELISA Kit employs a two-site sandwich ELISA to quantitate MTRR in samples. An antibody specific for MTRR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTRR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTRR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTRR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Methionine synthase reductase microplate
• Human Methionine synthase reductase standard
• Human Methionine synthase reductase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Methionine synthase reductase (MTRR) ELISA Kit has high sensitivity and excellent specificity for detection of Human MTRR. No significant cross-reactivity or interference between Human MTRR and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: MTRR encodes a member of the ferredoxin-NADP (+) reductase (FNR) family of electron transferases. Methionine synthase reductase functions in the synthesis of methionine by regenerating methionine synthase to a functional state. Because methionine synthesis requires methyl-group transfer by a folate donor, activity of the encoded enzyme is important for folate metabolism and cellular methylation. Mutations in MTRR can cause homocystinuria-megaloblastic anemia, cbl E type. Alternative splicing of MTRR results in multiple transcript variants.
Alternative Names: MTRR; MGC129643; MSR; cblE; [methionine synthase]-cobalamin methyltransferase (cob (II) alamin reducing) ; methionine synthase reductase
Search name: MTRR; MGC129643; MSR; cblE; [methionine synthase]-cobalamin methyltransferase (cob (II) alamin reducing) ; methionine synthase reductase
Tag: MTRR
