Euro
USD
British Pound
NULL
Product Description
Human Mitogen-activated protein kinase kinase kinase 6 (MAP3K6) ELISA Kit | KTE62235 | Abbkine
Application: This Human Mitogen-activated protein kinase kinase kinase 6 (MAP3K6) ELISA Kit employs a two-site sandwich ELISA to quantitate MAP3K6 in samples. An antibody specific for MAP3K6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAP3K6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP3K6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP3K6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Mitogen-activated protein kinase kinase kinase 6 microplate
• Human Mitogen-activated protein kinase kinase kinase 6 standard
• Human Mitogen-activated protein kinase kinase kinase 6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Mitogen-activated protein kinase kinase kinase 6 (MAP3K6) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAP3K6. No significant cross-reactivity or interference between Human MAP3K6 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: MAP3K6 is a member of the serine/threonine protein kinase family. The encoded kinase was identified by its interaction with MAP3K5/ASK, a protein kinase and an activator of c-Jun kinase (MAPK7/JNK) and MAPK14/p38 kinase. This kinase was found to weakly activate MAPK7, but not MAPK1/ERK or MAPK14. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined. MAP3K6 and MAP3K5 share 45% amino acid sequence identity; their catalytic domains share 82% identity. The authors confirmed the interaction of MAP3K6 with MAP3K5 in vivo using coimmunoprecipitation. Recombinant MAP3K6 weakly activated the JNK but not the ERK or p38 kinase pathways.
Alternative Names: MAP3K6; RP11-4K3__A.7; ASK2; MAPKKK6; MEKK6; MGC125653; MGC20114; apoptosis signal regulating kinase 2; apoptosis signal-regulating kinase 2
Search name: MAP3K6; RP11-4K3__A.7; ASK2; MAPKKK6; MEKK6; MGC125653; MGC20114; apoptosis signal regulating kinase 2; apoptosis signal-regulating kinase 2
Tag: MAP3K6
