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Product Description
Human Mucin-20 (MUC20) ELISA Kit | KTE61457 | Abbkine
Application: This Human Mucin-20 (MUC20) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC20 in samples. An antibody specific for MUC20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMUC20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC20 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Mucin-20 microplate
• Human Mucin-20 standard
• Human Mucin-20 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Mucin-20 (MUC20) ELISA Kit has high sensitivity and excellent specificity for detection of Human MUC20. No significant cross-reactivity or interference between Human MUC20 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: MUC20 encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The C-terminus of this family member associates with the multifunctional docking site of the MET proto-oncogene and suppresses activation of some downstream MET signaling cascades. The protein features a mucin tandem repeat domain that varies between two and six copies in most individuals. Multiple variants encoding different isoforms have been found for MUC20. A related pseudogene, which is also located on chromosome 3, has been identified.
Alternative Names: MUC20; FLJ14408; FLJ53153; KIAA1359; mucin 20; transmembrane mucin MUC20S
Search name: MUC20; FLJ14408; FLJ53153; KIAA1359; mucin 20; transmembrane mucin MUC20S
Tag: MUC20
