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Product Description
Human Mucosae associated epithelia chemokine (MEC) ELISA Kit | KTE62290 | Abbkine
Application: This Human Mucosae associated epithelia chemokine (MEC) ELISA Kit employs a two-site sandwich ELISA to quantitate CCL28 in samples. An antibody specific for CCL28 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCCL28 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL28 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL28 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Mucosae associated epithelia chemokine microplate
• Human Mucosae associated epithelia chemokine standard
• Human Mucosae associated epithelia chemokine detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Mucosae associated epithelia chemokine (MEC) ELISA Kit has high sensitivity and excellent specificity for detection of Human CCL28. No significant cross-reactivity or interference between Human CCL28 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: CCL28 is a chemokine. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 is expressed by columnar epithelial cells in the gut, lung, breast and the salivary glands and drives the mucosal homing of T and B lymphocytes that express CCR10, and the migration of eosinophils expressing CCR3. This chemokine is constitutively expressed in the colon, but its levels can be increased by pro-inflammatory cytokines and certain bacterial products implying a role in effector cell recruitment to sites of epithelial injury. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues.
Alternative Names: CCL28; CCK1; MEC; MGC71902; SCYA28; CC chemokine CCL28; mucosae-associated epithelial chemokine; small inducible cytokine A28; small inducible cytokine subfamily A (Cys-Cys) ; member 28
Search name: CCL28; CCK1; MEC; MGC71902; SCYA28; CC chemokine CCL28; mucosae-associated epithelial chemokine; small inducible cytokine A28; small inducible cytokine subfamily A (Cys-Cys) ; member 28
Tag: CCL28
