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Product Description
Human Myotubularin-related protein 6 (MTMR6) ELISA Kit | KTE61494 | Abbkine
Application: This Human Myotubularin-related protein 6 (MTMR6) ELISA Kit employs a two-site sandwich ELISA to quantitate MTMR6 in samples. An antibody specific for MTMR6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTMR6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTMR6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTMR6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Myotubularin-related protein 6 microplate
• Human Myotubularin-related protein 6 standard
• Human Myotubularin-related protein 6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Myotubularin-related protein 6 (MTMR6) ELISA Kit has high sensitivity and excellent specificity for detection of Human MTMR6. No significant cross-reactivity or interference between Human MTMR6 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: MTMR6 (Myotubularin Related Protein 6) is a Protein Coding gene. Diseases associated with MTMR6 include Myopathy, Centronuclear. Among its related pathways are Metabolism and PI Metabolism. GO annotations related to this gene include phosphatase activity and protein serine/threonine phosphatase activity. An important paralog of this gene is MTMR7. Phosphatase that acts on lipids with a phosphoinositol headgroup. Acts as a negative regulator of KCNN4/KCa3.1 channel activity in CD4+ T-cells possibly by decreasing intracellular levels of phosphatidylinositol 3 phosphatase. Negatively regulates proliferation of reactivated CD4+ T-cells.
Alternative Names: MTMR6; RP11-271M24.1;
Search name: MTMR6; RP11-271M24.1;
Tag: MTMR6
