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Product Description
Human Paraneoplastic antigen Ma2 (PNMA2) ELISA Kit | KTE62206 | Abbkine
Application: This Human Paraneoplastic antigen Ma2 (PNMA2) ELISA Kit employs a two-site sandwich ELISA to quantitate PNMA2 in samples. An antibody specific for PNMA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPNMA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PNMA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PNMA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Paraneoplastic antigen Ma2 microplate
• Human Paraneoplastic antigen Ma2 standard
• Human Paraneoplastic antigen Ma2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Paraneoplastic antigen Ma2 (PNMA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PNMA2. No significant cross-reactivity or interference between Human PNMA2 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: In patients with cancer, symptoms of limbic and brainstem dysfunction may result from a paraneoplastic disorder. Paraneoplastic limbic or brainstem encephalitis occurs more frequently with testicular cancer than with most other cancers. By immunohistochemical and immunoblotting techniques, Voltz et al. (1999) detected antibodies in serum and cerebrospinal fluid against a 40-kD neuronal protein in 10 of 13 patients with testicular cancer and paraneoplastic limbic or brainstem encephalitis, or both. They designated the protein MA2, and found that it was selectively expressed by normal brain tissue and by the testicular tumors of the patients. MA2 shares homology with MA1, a 'brain-testis-cancer' gene related to other neoplastic syndromes and tumors.
Alternative Names: PNMA2; KIAA0883; MA2; MM2; RGAG2; onconeuronal antigen MA2; paraneoplastic neuronal antigen; retrotransposon gag domain containing 2
Search name: PNMA2; KIAA0883; MA2; MM2; RGAG2; onconeuronal antigen MA2; paraneoplastic neuronal antigen; retrotransposon gag domain containing 2
Tag: PNMA2
