Product Description
Human Perilipin-4 (KIAA1881) ELISA Kit | KTE61921 | Abbkine
Application: This Human Perilipin-4 (KIAA1881) ELISA Kit employs a two-site sandwich ELISA to quantitate KIAA1881 in samples. An antibody specific for KIAA1881 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKIAA1881 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KIAA1881 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIAA1881 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Perilipin-4 microplate
• Human Perilipin-4 standard
• Human Perilipin-4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Perilipin-4 (KIAA1881) ELISA Kit has high sensitivity and excellent specificity for detection of Human KIAA1881. No significant cross-reactivity or interference between Human KIAA1881 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Members of the perilipin family, such as PLIN4, coat intracellular lipid storage droplets The predicted 1, 348-amino acid protein is homologous to the mouse plasma membrane-associated protein S3-12. RT-PCR ELISA detected ubiquitous expression of PLIN4, with highest expression in skeletal muscle, followed by heart and liver, and lowest expression in adult whole brain. Using immunofluorescence microscopy, Wolins et al. (2003) demonstrated that supplementation of mouse adipocytes with fatty acids resulted in a shift from diffuse S3-12 cytoplasmic expression to S3-12 coating of lipid droplets on the cell periphery. Over time, Plin1 coexpression was observed. Formation of S3-12-coated droplets required glucose and fatty acids that could be incorporated into triacylglycerol and was dependent on insulin.
Alternative Names: KIAA1881
Search name: KIAA1881
Tag: KIAA1881