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Product Description
Human Plasmalemma vesicle-associated protein (PLVAP) ELISA Kit | KTE62358 | Abbkine
Application: This Human Plasmalemma vesicle-associated protein (PLVAP) ELISA Kit employs a two-site sandwich ELISA to quantitate PLVAP in samples. An antibody specific for PLVAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLVAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLVAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLVAP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Plasmalemma vesicle-associated protein microplate
• Human Plasmalemma vesicle-associated protein standard
• Human Plasmalemma vesicle-associated protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Plasmalemma vesicle-associated protein (PLVAP) ELISA Kit has high sensitivity and excellent specificity for detection of Human PLVAP. No significant cross-reactivity or interference between Human PLVAP and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: PLVAP is a type II integral membrane protein with a short N-terminal cytoplasmic tail, a single-span transmembrane domain, and a large C-terminal extracellular domain consisting mostly of alpha helices. It also contains 2 coiled-coil domains predicted to contribute to dimer formation. The extracellular domain has 4 N-glycosylation sites, a proline-rich region, and regular spacing of cysteine residues. The deduced 439-amino acid mouse Plvap protein contains a consensus casein kinase II phosphorylation site that is not found in the human protein. Rat Plvap contains 2 casein kinase II phosphorylation sites. Northern blot analysis revealed a single PLVAP transcript of about 2.4 kb in pancreas, kidney, placenta, muscle, lung, heart, and liver. No signal was detected in brain or testis.
Alternative Names: PLVAP; FELS; PV-1; PV1; gp68; fenestrated-endothelial linked structure protein; PV-1 protein
Search name: PLVAP; FELS; PV-1; PV1; gp68; fenestrated-endothelial linked structure protein; PV-1 protein
Tag: PLVAP
