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Product Description
Human Protein FAM3B (FAM3B) ELISA Kit | KTE62394 | Abbkine
Application: This Human Protein FAM3B (FAM3B) ELISA Kit employs a two-site sandwich ELISA to quantitate FAM3B in samples. An antibody specific for FAM3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFAM3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FAM3B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FAM3B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Protein FAM3B microplate
• Human Protein FAM3B standard
• Human Protein FAM3B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Protein FAM3B (FAM3B) ELISA Kit has high sensitivity and excellent specificity for detection of Human FAM3B. No significant cross-reactivity or interference between Human FAM3B and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: The authors predicted that FAM3B forms a 4-helix bundle, and 3-dimensional modeling indicated that 4 cysteines could form 2 disulfide bonds linking helices 1 and 4 and helices 2 and 3. The human and mouse FAM3B proteins share 78% amino acid identity. Northern blot analysis detected FAM3B expression at a high level in pancreas and at lower levels in small intestine and prostate. Immunohistochemical analysis of mouse tissues detected prominent Fam3b staining in the endocrine pancreas, specifically in the islets of Langerhans. Staining was also found in round spermatids within the seminiferous tubules, in the Purkinje cell layer of the cerebellum, and in nerve cell bodies of numerous brainstem nuclei. Transfected Chinese hamster ovary cells secreted Fam3b to the culture medium.
Alternative Names: FAM3B; 2-21; C21orf11; C21orf76; ORF9; PANDER; PRED44; D21M16SJHU19e; cytokine-like protein 2-21; pancreatic derived factor
Search name: FAM3B; 2-21; C21orf11; C21orf76; ORF9; PANDER; PRED44; D21M16SJHU19e; cytokine-like protein 2-21; pancreatic derived factor
Tag: FAM3B
