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Product Description
Human Protein lin-28 homolog A (LIN28) ELISA Kit | KTE61811 | Abbkine
Application: This Human Protein lin-28 homolog A (LIN28) ELISA Kit employs a two-site sandwich ELISA to quantitate LIN28 in samples. An antibody specific for LIN28 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLIN28 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LIN28 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LIN28 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Protein lin-28 homolog A microplate
• Human Protein lin-28 homolog A standard
• Human Protein lin-28 homolog A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Protein lin-28 homolog A (LIN28) ELISA Kit has high sensitivity and excellent specificity for detection of Human LIN28. No significant cross-reactivity or interference between Human LIN28 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: LIN28 protein is stage-specifically expressed in embryonic muscle, neurons, and epithelia, as well as in embryonic carcinoma cells; however, it is suppressed in most adult tissues except for skeletal and cardiac muscle.LIN28 mRNA is a target of the micro RNAs miR-125b and miRNA-125a.When the miREs were deleted, miRNA control over LIN28 nearly vanished. Lin28 downregulation by miR-125 involved reductions in both translational efficiency and mRNA abundance.An important target of Lin28 is Igf2, a growth and differentiation factor for muscle tissue. Interaction of Lin28 with translation initiation complexes in skeletal myoblasts and in the embryonic carcinoma cell line P19 was confirmed by localization of Lin28 to the stress granules, temporary structures that contain stalled mRNA-protein translation complexes.
Alternative Names: LIN28; CSDD1; FLJ12457; LIN-28; LIN28A; ZCCHC1; RNA-binding protein LIN-28; lin-28 homolog; zinc finger; CCHC domain containing 1
Search name: LIN28; CSDD1; FLJ12457; LIN-28; LIN28A; ZCCHC1; RNA-binding protein LIN-28; lin-28 homolog; zinc finger; CCHC domain containing 1
Tag: LIN28
