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Product Description
Human Putative beta-glucuronidase-like protein SMA3 (GUSBP1) ELISA Kit | KTE62388 | Abbkine
Application: This Human Putative beta-glucuronidase-like protein SMA3 (GUSBP1) ELISA Kit employs a two-site sandwich ELISA to quantitate GUSBP1 in samples. An antibody specific for GUSBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGUSBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GUSBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GUSBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Putative beta-glucuronidase-like protein SMA3 microplate
• Human Putative beta-glucuronidase-like protein SMA3 standard
• Human Putative beta-glucuronidase-like protein SMA3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Putative beta-glucuronidase-like protein SMA3 (GUSBP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human GUSBP1. No significant cross-reactivity or interference between Human GUSBP1 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Beta-glucuronidases are members of the glycosidase family of enzymes that catalyze breakdown of complex carbohydrates. Human β-glucuronidase is a type of glucuronidase (a member of glycosidase Family 2) that catalyzes hydrolysis of β-D-glucuronic acid residues from the non-reducing end of mucopolysaccharides (also referred to as glycosaminoglycans) such as heparan sulfate. Human β-glucuronidase is located in the lysosome. In the gut, brush border β-glucuronidase converts conjugated bilirubin to the unconjugated form for reabsorption. Beta-glucuronidase is also present in breast milk, which contributes to neonatal jaundice. The protein is encoded by the GUSB gene.
Alternative Names: GUSBP1; SMA3;
Search name: GUSBP1; SMA3;
Tag: GUSBP1
